Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
نویسندگان
چکیده
منابع مشابه
Haplotype and mutation analysis for newly suggested Y-STRs in Korean father-son pairs.
In this study, 363 Korean father-son haplotype transfers in 351 families were analyzed using an in-house multiplex PCR system for 14 Y-STRs (DYS385a/b, DYF387S1, DYS391, DYS449, DYS460, DYS481, DYS518, DYS533, DYS549, DYS570, DYS576, DYS627 and DYS643), that included 11 loci newly added to the PowerPlex Y23 system or the Yfiler Plus system. The Y-STRs showed gene diversity values ranging from 0...
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Next-generation sequencing (NGS) machines can sequence millions of DNA strands in a single run, such as oligonucleotide (oligo) libraries comprising millions to trillions of discrete oligo sequences. Capillary electrophoresis is an attractive technique to select tight binding oligos or "aptamers" because it requires minimal sample volumes (e.g., 100 nL) and offers a solution-phase selection env...
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22 23 Y-chromosome microsatellites (Y-STRs) are typically used for kinship analysis and 24 forensic identification as well as for inferences on population history and evolution. All 25 applications would greatly benefit from reliable locus-specific mutation rates, to improve 26 forensic probability calculations and interpretations of diversity data. However, estimates of 27 mutation rate from f...
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Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
متن کاملStrategies and Clinical Applications of Next Generation Sequencing
Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
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ژورنال
عنوان ژورنال: Forensic Sciences Research
سال: 2021
ISSN: 2096-1790,2471-1411
DOI: 10.1080/20961790.2021.1898078